Friends, this image was part of my presentation on focus- stacking of multiple images.  These beads are 1/8-1/4 inch size so this was a macro photo.  To get the desired magnification, i had to have the lens within a foot of the front surface of the beads. However, the depth of field would not extend to back of image, so took 12 images focusing stepwise from front to back, then stitched the images together with Photoshop, to get this image that is in focus from front to back.  til Tomorrow MJ